complete a549 media Search Results


a549 cells  (ATCC)
99
ATCC a549 cells
A549 Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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complete a549 media  (ATCC)
99
ATCC complete a549 media
NSCLC growth is dependent on matrix type and stiffness. (A) Viability staining of encapsulated <t>A549</t> cells using fluorescein diacetate (FDA, green, live) and propidium iodide (PI, red, dead). Day 1 scale = 200 μm, day 7 and 14 scales = 500 200 μm. (B) Representative fluorescent images of A549-laden cultures fixed on days 3, 7, and 14 and stained with DAPI (blue, nuclei) and phalloidin (red, actin) to observe cell morphology and distribution over time. Scale = 250 μm, inset scale = 100 μm. (C) Cell viability of A549s within matrices measured through live-dead quantification of FDA or PI stained live (green) and dead (red) cells over time via ImageJ analysis. N = 1. n = 3. (D) Metabolic activity of A549 cells within matrices was determined by PrestoBlue metabolic assay. N = 1. n = 6. (E) DNA content of A549 cells within matrices was determined by PicoGreen™ DNA quantification. N = 1. n = 6. (F) Number of nuclei per ROI was determined via Image J analysis of DAPI-stained cells within ROIs captured using an Evident FV4000 confocal microscope at 10× magnification. N = 2. n = 4–8 ROIs were captured per condition per timepoint. (G) Area of actin per ROI (μm 2 ) was determined through ImageJ analysis of phalloidin-stained cell actin fibers. N = 2. n = 4–8. All data is represented as mean ± SD.
Complete A549 Media, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 99 stars, based on 1 article reviews
complete a549 media - by Bioz Stars, 2026-02
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dmem-high glucose  (Millipore)
90
Millipore dmem-high glucose
NSCLC growth is dependent on matrix type and stiffness. (A) Viability staining of encapsulated <t>A549</t> cells using fluorescein diacetate (FDA, green, live) and propidium iodide (PI, red, dead). Day 1 scale = 200 μm, day 7 and 14 scales = 500 200 μm. (B) Representative fluorescent images of A549-laden cultures fixed on days 3, 7, and 14 and stained with DAPI (blue, nuclei) and phalloidin (red, actin) to observe cell morphology and distribution over time. Scale = 250 μm, inset scale = 100 μm. (C) Cell viability of A549s within matrices measured through live-dead quantification of FDA or PI stained live (green) and dead (red) cells over time via ImageJ analysis. N = 1. n = 3. (D) Metabolic activity of A549 cells within matrices was determined by PrestoBlue metabolic assay. N = 1. n = 6. (E) DNA content of A549 cells within matrices was determined by PicoGreen™ DNA quantification. N = 1. n = 6. (F) Number of nuclei per ROI was determined via Image J analysis of DAPI-stained cells within ROIs captured using an Evident FV4000 confocal microscope at 10× magnification. N = 2. n = 4–8 ROIs were captured per condition per timepoint. (G) Area of actin per ROI (μm 2 ) was determined through ImageJ analysis of phalloidin-stained cell actin fibers. N = 2. n = 4–8. All data is represented as mean ± SD.
Dmem High Glucose, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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cd95 mediated apoptosis  (ATCC)
96
ATCC cd95 mediated apoptosis
NSCLC growth is dependent on matrix type and stiffness. (A) Viability staining of encapsulated <t>A549</t> cells using fluorescein diacetate (FDA, green, live) and propidium iodide (PI, red, dead). Day 1 scale = 200 μm, day 7 and 14 scales = 500 200 μm. (B) Representative fluorescent images of A549-laden cultures fixed on days 3, 7, and 14 and stained with DAPI (blue, nuclei) and phalloidin (red, actin) to observe cell morphology and distribution over time. Scale = 250 μm, inset scale = 100 μm. (C) Cell viability of A549s within matrices measured through live-dead quantification of FDA or PI stained live (green) and dead (red) cells over time via ImageJ analysis. N = 1. n = 3. (D) Metabolic activity of A549 cells within matrices was determined by PrestoBlue metabolic assay. N = 1. n = 6. (E) DNA content of A549 cells within matrices was determined by PicoGreen™ DNA quantification. N = 1. n = 6. (F) Number of nuclei per ROI was determined via Image J analysis of DAPI-stained cells within ROIs captured using an Evident FV4000 confocal microscope at 10× magnification. N = 2. n = 4–8 ROIs were captured per condition per timepoint. (G) Area of actin per ROI (μm 2 ) was determined through ImageJ analysis of phalloidin-stained cell actin fibers. N = 2. n = 4–8. All data is represented as mean ± SD.
Cd95 Mediated Apoptosis, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


NSCLC growth is dependent on matrix type and stiffness. (A) Viability staining of encapsulated A549 cells using fluorescein diacetate (FDA, green, live) and propidium iodide (PI, red, dead). Day 1 scale = 200 μm, day 7 and 14 scales = 500 200 μm. (B) Representative fluorescent images of A549-laden cultures fixed on days 3, 7, and 14 and stained with DAPI (blue, nuclei) and phalloidin (red, actin) to observe cell morphology and distribution over time. Scale = 250 μm, inset scale = 100 μm. (C) Cell viability of A549s within matrices measured through live-dead quantification of FDA or PI stained live (green) and dead (red) cells over time via ImageJ analysis. N = 1. n = 3. (D) Metabolic activity of A549 cells within matrices was determined by PrestoBlue metabolic assay. N = 1. n = 6. (E) DNA content of A549 cells within matrices was determined by PicoGreen™ DNA quantification. N = 1. n = 6. (F) Number of nuclei per ROI was determined via Image J analysis of DAPI-stained cells within ROIs captured using an Evident FV4000 confocal microscope at 10× magnification. N = 2. n = 4–8 ROIs were captured per condition per timepoint. (G) Area of actin per ROI (μm 2 ) was determined through ImageJ analysis of phalloidin-stained cell actin fibers. N = 2. n = 4–8. All data is represented as mean ± SD.

Journal: Materials Today Bio

Article Title: Photocrosslinkable lung dECM hydrogels promote stiffness-dependent lung cancer growth and chemoresistance

doi: 10.1016/j.mtbio.2026.102838

Figure Lengend Snippet: NSCLC growth is dependent on matrix type and stiffness. (A) Viability staining of encapsulated A549 cells using fluorescein diacetate (FDA, green, live) and propidium iodide (PI, red, dead). Day 1 scale = 200 μm, day 7 and 14 scales = 500 200 μm. (B) Representative fluorescent images of A549-laden cultures fixed on days 3, 7, and 14 and stained with DAPI (blue, nuclei) and phalloidin (red, actin) to observe cell morphology and distribution over time. Scale = 250 μm, inset scale = 100 μm. (C) Cell viability of A549s within matrices measured through live-dead quantification of FDA or PI stained live (green) and dead (red) cells over time via ImageJ analysis. N = 1. n = 3. (D) Metabolic activity of A549 cells within matrices was determined by PrestoBlue metabolic assay. N = 1. n = 6. (E) DNA content of A549 cells within matrices was determined by PicoGreen™ DNA quantification. N = 1. n = 6. (F) Number of nuclei per ROI was determined via Image J analysis of DAPI-stained cells within ROIs captured using an Evident FV4000 confocal microscope at 10× magnification. N = 2. n = 4–8 ROIs were captured per condition per timepoint. (G) Area of actin per ROI (μm 2 ) was determined through ImageJ analysis of phalloidin-stained cell actin fibers. N = 2. n = 4–8. All data is represented as mean ± SD.

Article Snippet: 1 × 10 6 A549 cells (ATCC) were seeded at P90 in T75 culture flasks and cultured using 10 mL complete A549 media (Dulbecco's modified eagle medium (DMEM) + 10 % FCS + 1 % pen/strep) in a tissue culture incubator at 37 °C.

Techniques: Staining, Activity Assay, Metabolic Assay, Microscopy